Sabtu, 19 Desember 2015

Deployment of Improved Genetic Material to increase plantation forest productivity


1.  Objective

To provide guidance for deploying improved materials, so that the materials can well perform as per expectation in plantation compartments.

2.  Genetic Deployment Principles

2.1.    It is not possible to directly observe and control most genes (inheritance factors) affecting plantation productivity.  Therefore, it is not appropriate for just simply picking and fixing the best materials for deployment purpose.

2.2.    Consequently, proper determination of Experimental Model, Analysis of Variance, Variance Component Analysis and Expected Gain Calculation is mandatory to be used for the basis of determining improved genetic material for Deployment.

2.3.    The areas under the plantation program are very diverse in geology, soils, terrains and other landscape attributes which have equally diverse opportunities and constrains for plantation productivity.  Therefore, proper stage of evaluation through gradual increase in size of testing area, is mandatory to anticipate possible differences in productivity in such diverse range of sites.

2.4.    “C” Effect Variance, i.e. Non Genetic Variance imposed by Physiological Maturity or Propagation Method can give great impact on reducing potential productivity of genetic materials. Hence, clear statement of propagule type used during the various stage of testing is mandatory to facilitate assessment on possible occurrence of the “C” Effect variance.

3.  Procedure for Deploying Improved Genetic Materials

Each improved genetic material shall go through four stages of testing (see point 3.1. below) followed by proper genetic analysis (point 3.2. below), prior to deployment for commercial plantation.




3.1. Testing Stage

Four testing stages that shall be made for improved genetic material include: Basic Test, Intermediate Test, Pre-Commercial Test and Commercial Test. The main difference between these tests is size of total plot area, being the smallest total plot area is Basic Test with minimum total plot area of 0.1 hectare, and the largest plot area is Commercial Test with minimum total plot area of 90 hectare (see Table 1 below)

Results of the four testing stage shall be summarized in Table 1 as shown below:


Table 1.   Results of Basic, Intermediate, Pre-Commercial, and Commercial Tests of  improved genetic materials for Deployment.

Deploment ID: 
………………..








Type of Test
Type of Propagule Used
Plot Size (tree)
Spacing (m)
Area/ Plot (ha)
No of Replicate
No of Site
Total Plot Area (ha)
Age of Assessment (year)
Expected Gain
Basic

10
3 x 2
0.01
8
2
0.10
                     3

Intermediate

49
3 x 2
0.03
5
2
0.29
                     3

Pre-Commercial

1,667
3 x 2
1
3
3
9
                     3

Commercial

16,667
3 x 2
10
3
3
90
                     3


The summary of the above Table shall be accompanied with Genetic Analysis Results (see point 3.2 below)

3.2. Genetic Analysis

Genetic analysis comprises Determination of Experimental Model, Analysis of Variance, Variance Component Analysis, Heritability and Expected Gain Calculation, with an example of general guidance is briefly shown below:

3.2.1. Experimental Model

Experimental Model for Multi Observation Data (Multi Observation Data : when a single character from the same replication is measured more than once):

Yijk = m + Ri + Fj+ RFij + dijk

Yijk
:
Data Values (data values from each measurement)
m
:
Overall Mean (Base Line)
Ri
:
Effects of Replication
Fj
:
Effects of Families
RFij
:
Interaction between Replications and Families
dijk
:
Trees within Plot


3.2.2. Estimation of Terms in Experimental Model

µ
:
Y...
Ri
:
Yi.. - µ
Fj
:
Y.j. - µ
RFij
:
Yij. -  µ   - Ri - Fj
dijk
:
Yijk -  µ - Ri - Fj - RFij

3.2.3. Analysis of Variance 

 




3.2.4. Variance Component Analysis


 
a.       Families x replications


































b.      Families



































c.       Phenotypic Variance
 
























3.2.5. Heritability

















3.2.6. Individual Tree Heritability
                                                                                  
 

3.2.7. Family Heritability
 


3.2.7. Gain